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Image Search Results
Journal: EMBO Molecular Medicine
Article Title: Comparative proteomics reveals a diagnostic signature for pulmonary head‐and‐neck cancer metastasis
doi: 10.15252/emmm.201708428
Figure Lengend Snippet: A Volcano plot relating adjusted p values for differential protein expression to averaged normalised SILAC ratios of two replicates. Red (higher expression in HNSCC) and blue (higher expression in SQCLC) dots indicate significantly regulated proteins ( P < 0.05). B Two‐class comparison of genetic dependencies from a publically available genome‐scale CRISPR‐Cas9 screen of HNSCC (12 cell line) versus SQCLC (10 cell lines) identified a subset of differentially expressed proteins that were also differential dependencies in these tumour types. The x ‐axis represents the effect size of the mean difference of dependency scores in HNSCC compared to SQCLC cell lines. Positive effect size indicates a greater mean dependency in HNSCC; negative effect size indicates a greater mean dependency in SQCLC. The y ‐axis represents the statistical significance of differential enrichment calculated as ‐log10( P ‐value) from a two‐sided t ‐test. The P ‐values used for this plot are uncorrected for multiple hypothesis testing. Highlighted in green are genetic dependencies that were also identified as differentially expressed proteins in our study. C, D Immunohistochemical analysis of the expression of HMGCS‐1 ( P = 0.0014), FTL ( P = 0.0001) (C), LGALS7 ( P = 0.0001) and FAM83H ( P = 0.0001) (D) in an independent cohort of 212 SQCLC and 343 HNSCC cases. Scale bar indicates 100 μm. Shown are mean values and standard deviation. Statistical significance was assessed using Wilcoxon–Mann–Whitney test. E Pathway enrichment analysis for proteins differentially expressed in HNSCC and SQCLC.
Article Snippet: Briefly, 2‐μm tissue sections were incubated in EnVision Flex Target Retrieval Solution, pH high or low (Dako) followed by incubation of primary antibodies against CK5/6 (Dako, prediluted, high), CK7 (Dako, prediluted, high), p63 (Bio‐Genex, prediluted, high), TTF‐1 (Dako, prediluted, high), CD34 (Dako, prediluted, low), CK19 (Dako, prediluted, high),
Techniques: Expressing, Multiplex sample analysis, Comparison, CRISPR, Immunohistochemical staining, Standard Deviation, MANN-WHITNEY